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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Science. 2017 Jan 20;355(6322):298–302. doi: 10.1126/science.aah6130

Fig. 4. Pathways of TA protein flux through the triage system.

Fig. 4

(A) Photocrosslinking timecourse (top) and quantification (bottom) of TA(BpF) (Sec61β) transfer from SGTA to the Bag6 complex (B/U/T) without (n=3) or with (n=1) 10X CaM. Where applicable, mean ± SEM values are displayed with a first-order exponential decay fit. The gray dotted curve refers to the rate of spontaneous TA protein release from SGTA to 10X CaM from Fig. 3B. (B) Photocrosslinking timecourse (top) and quantification (bottom) of TA(BpF) (Sec61β) transfer from SGTA to TRC40 via cB/U/T without (n=3) or with (n=1) 10X CaM. (C) TA(BpF) (Sec61β) in complex with SGTA (top) or CaM (bottom) was incubated with the indicated factors and analyzed for TMD interactions at various times by photocrosslinking. (D) Photocrosslinking timecourse of TA(BpF) (Sec61β) transfer from SGTA in the presence of the full Bag6 complex and TRC40 without or with 10X CaM. (E) Direct comparison of TA (BpF) photocrosslinking interactions in the complete triage reaction after 5 min without (n=3 used for quantification) or with 10X CaM (n=2). The extent of TA protein interaction with Bag6 and TRC40 was quantified (right; mean ± SEM). All reactions contained 750 nM of each TRC factor.

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