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. 2017 Apr 4;12(4):e0174985. doi: 10.1371/journal.pone.0174985

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© 2017 Mitsuishi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — (A) The surface layer of a tumor (HE staining), showing hyperplasia of small atypical ducts and dilated atypical ducts. (B) The surface layer of a tumor (MUC5AC antibody staining), showing the presence of MUC5AC-positive cells in the superficial layer of the tumor. (C) The surface layer of a tumor (MUC6 antibody staining), showing the presence of MUC6-positive cells in a large area, excluding the superficial layer of the tumor. (D) The deep region of a tumor (HE staining), showing hyperplasia of small atypical ducts. A region showing hyperplasia of clear cells with no atypia was found in the deepest part of the tumor. (E) A deep part of a tumor (MUC6 antibody staining), showing the presence of MUC6-positive cells in a large area extending from the atypical ducts to the region of clear-cell hyperplasia. (F) A magnified image of a tumor (HE staining), showing a distinctly protruding lesion. A region of tumor duct and mucous duct hyperplasia was found in the lamina propria. The muscularis mucosa was compressed and pushed down deeply.