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. 2017 Apr 4;6:e23008. doi: 10.7554/eLife.23008

Figure 4. Representative NanoSIMS ion images of Symbiodinium cells exposed to 34S- or natS-artificial seawater (ASW) for 18 days and subsequently inoculated with two different bacterial strains for six hours.

(a) Timeline of the experiment. (b, c and d) 12C15N/12C14N mass images showing the presence of 15N enriched bacterial cells. (e, f and g) 34S/32S ratio image of the same regions. These mass images are shown as HSI images where the colour scale indicates the value of the stable isotope ratios, with natural abundance in blue, changing to pink with increasing 15N or 34S levels. (b, c, e and f) Symbiodinium cultures were inoculated with the DMSP-degrading bacterium Pseudovibrio sp. P12 (treatment 1). (d and g) Symbiodinium cultures were inoculated with Escherichia coli (treatment 2). White arrows indicate bacteria. (h) Isotope ratio of 34S/32S in bacteria, Pseudovibrio cells were significantly more enriched than E. coli (t-Test, n = 60, t = 9.021, *p<0.001, error bars: SE). The dashed blue line represents the natural 34S abundance recorded in the control samples. (i) Total particulate DMSP concentration in Symbiodinium inoculated with Pseudovibrio sp. or E. coli (t-Test, n = 3, t = 9.908, *p<0.001, error bar: SE). Source data available: Figure 4—source data 1. Note: two regions of interest were merged to create Figure 4c due to stage-shifting errors during sequential acquisition of N and S data. Scale bars = 3 µm.

DOI: http://dx.doi.org/10.7554/eLife.23008.014

Figure 4—source data 1. 12C15N, 12C14N, 32S and 34S measured in the different organisms and treatments depicted in Figure 4h and Figure 4—figure supplement 1.
DOI: http://dx.doi.org/10.7554/eLife.23008.015
elife-23008-fig4-data1.xlsx (66.7KB, xlsx)
DOI: 10.7554/eLife.23008.015

Figure 4.

Figure 4—figure supplement 1. Isotope ratio of (a) 15N/14N and (b) 34S/32S in Symbiodinium and bacteria cells measured by NanoSIMS in the different treatments (values were extracted from the images).

Figure 4—figure supplement 1.

The dashed lines represent the natural 15N and 34S abundance measured in the controls (0.0037 and 0.0438, respectively). 15N/14N of the inoculated bacterial cells was 4.2243 ± 0.1886 on average, compared to 0.0037 ± 8.29E-06 recorded in the controls. Source data available: Figure 4—source data 1. Note: only inoculated bacteria were incubated in 15N. Error bars = SE.
DOI: http://dx.doi.org/10.7554/eLife.23008.016