Figure 6. SrrAB-dependent biofilm formation is responsive to the oxidation state of the cellular menaquinone pool.
Panel A; SrrAB-dependent biofilm formation is inactivated in strains lacking the ability to synthesize menaquinone. Biofilm formation following aerobic growth is displayed for the menF::Tn (JMB6219), hemB::Tn (JMB6037), ΔsrrAB menF::Tn (JMB6221), ΔsrrAB hemB::Tn (JMB6039), hemB::Tn menF::Tn (JMB6217), and ΔsrrAB hemB::Tn menF::Tn (JMB6673) strains. Panel B; SrrAB-dependent biofilm formation is not stimulated in strains enriched for oxidized menaquinone. Biofilm formation following aerobic growth is displayed for the WT (JMB 1100), ΔsrrAB (JMB 1467), ΔndhC ndhF::Tn sdh:Tn (JMB 6613), and ΔsrrAB ΔndhC ndhF::Tn sdh:Tn (JMB 6614) strains. Data in both panels represent the average value of eight wells and the errors bars represent standard deviation. Statistical significance was calculated using a two-tail Student's t-test and p-values>0.05 were considered to be not significant while * indicates p-value of <0.05.