Figure 1. 14-3-3σ contributes to cellular resistance to DNA-damaging drugs and IR.
(A) Western blot analysis of 14-3-3σ in stable MiaPaCa-2/Vec and MiaPaCa-2/σ and in BxPC-3/Scr and BxPC-3/Sh-σ cells. (B) MTT assay of survival against different therapeutic drugs. GEM, gemcitabine; MTX, mitoxantrone; DOX, doxorubicin. Relative resistance factor (REF)=IC50(MiaPaCa-2/σ)/IC50(MiaPaCa-2/Vec) or IC50(BxPC-3/Sh-σ)/IC50(BxPC-3/Scr). (C) Colony formation assay of survival against IR. (*p<0.05; **p<0.01; n=3).