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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Cancer Res. 2017 Jan 13;15(4):418–428. doi: 10.1158/1541-7786.MCR-16-0366

Figure 7. 14-3-3σ regulation of G2/M arrest and Chk2 expression.

Figure 7

(A–B) Cell cycle distribution of MiaPaCa-2/σ and BxPC-3/Sh-σ in comparison with their respective control cells following IR. (C–D) Western blot (C) and real time RT-PCR (D) analysis of Chk2 expression in MiaPaCa-2/σ and BxPC-3/Sh-σ in comparison with their respective control cells. (E–F) Western blot (E) and real-time RT-PCR (F) analyses of Chk2 knockdown by siRNAs. (G) FACS analysis of Chk2 knockdown on cell cycle distribution following IR in MiaPaCa-2/σ cells. (H) Host cell reactivation assay of the effect of Chk2 knockdown on 14-3-3σ-induced NHEJ activity increase. (*p<0.05; **p<0.01; n=3).