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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Cancer Res. 2016 Nov 9;23(7):1809–1819. doi: 10.1158/1078-0432.CCR-16-1818

Figure 3. Changes in anti-viral signaling in macrophages co-cultured with glioma infected with RAMBO Vs rHSVQ1.

Figure 3

Comparison of murine macrophage anti-viral gene signaling when cultured with rHSVQ1 or RAMBO-infected glioma cells. Briefly, U251 human glioma cells infected with PBS, rHSVQ1 or RAMBO were overlaid with murine RAW macrophage cells. 12 hours post infection, we analyzed changes in murine specific cytokine gene expression using an antiviral gene expression PCR array. (A) Volcano plot comparing normalized changes in gene expression of macrophages cultured with glioma cells treated with rHSVQ1 vs. uninfected (left panel), RAMBO vs. uninfected (middle panel), and RAMBO vs. rHSVQ1 (right panel) using 1.5-fold gene change as cut-off, and p≤0.05. (B) Venn diagrams of macrophage genes induced upon infection with rHSVQ1 and/or RAMBO (left panel), induced by both rHSVQ1 and RAMBO relative to uninfected (middle panel) and down regulated by rHSVQ1 and/or RAMBO (right panel). (C) Ingenuity Pathway Analysis of genes showing a differentially induction (≥ 1.5 fold) in macrophages in response to rHSVQ1 vs. RAMBO infected cells. The network is graphically-represented as nodes (genes); bold lines connecting the nodes indicate direct interaction, while the dashed lines suggest indirect interaction