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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Clin Cancer Res. 2016 Nov 9;23(7):1809–1819. doi: 10.1158/1078-0432.CCR-16-1818

Figure 5. Reduced TNFα upon RAMBO infection depends on BAI1 expression in macrophages.

Figure 5

Primary bone marrow-derived murine macrophages from Bai+/+ or Bai1−/− mice were utilized to evaluate the impact of Bai1 on viral replication in co-culture with U251 glioma cells. (A) Twelve hours post infection, changes in viral ICP4 gene expression was compared between rHSVQ1 or RAMBO infected cells cultured with Bai1+/+ or Bai1−/− macrophages. Data shown is mean fold-change in ICP4 gene expression ± s.d., normalized to levels in rHSVQ1/Bai1+/+ co-culture. (B) Twelve hours post infection, changes in viral replication was compared between rHSVQ1- or RAMBO-infected U251 glioma cells cultured with Bai1+/+ or Bai1−/− macrophages. Data shown is mean replication (plaque-forming units/mL) ± s.d. (C) Change in murine TNFα gene expression was measured 12h after co-culture with infected U251 glioma cells. Data shown is relative TNFα gene expression ± s.d., normalized to expression in Bai1+/+ co-cultured with rHSVQ1. (D) Wild-type HSV-1 (F strain) was injected into naïve non-tumor-bearing brains of Bai1−/− or Bai1+/+ mice. Data shown is Kaplan-Meier survival curve. Mice were euthanized when they showed symptoms of viral encephalitis, including hunched posture, rough coat, thin body, or limb paralysis. (*, p≤0.05; **, p≤0.01; ****, p≤0.0001)