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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Mol Cancer Res. 2017 Jan 4;15(4):478–488. doi: 10.1158/1541-7786.MCR-16-0337

Figure 1. Elevated OSM and OSMR in PDAC drive EMT.

Figure 1

(A) Oncomine data of whole tumor tissue vs. normal pancreas tissue examining OSM and OSMR mRNA levels in PDAC and normal pancreas (60). (B) Expression of a STAT3 gene expression signature (generated by filtering Pancreatic Cancer vs. Normal analysis; then applying filter “genes differentially expressed in melanoma cells in response to STAT3 expression”) in PDAC and normal pancreas. (C–E) A panel of PDAC cells lines were treated with recombinant OSM for one week and analyzed for morphological changes using bright-field microscopy (D; images shown at 200X). STAT3 phosphorylation (Tyr 705), total STAT3, and Actin were assessed using Western blot analysis (D), and E-Cadherin (CDH1) and ZEB1 were assessed using qRT-PCR (E).