Figure 6. ZEB1 is crucial to OSM-induced mesenchymal/CSC plasticity.
CD24LOW/CD44HI HPAC-OSM cells were infected with lentiviruses encoding two short hairpin RNAs targeting ZEB1 (shZEB1-765 and shZEB1-766) or a control (shGFP). Following selection, Western blot analysis (A), qRT-PCR (B), bright-field microscopy (C) and flow cytometry (D) were performed as indicated.