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Quantitative analysis of interferon (IFN)-γ- and interleukin (IL)-4-producing cells. On day 42, mice (n = 6) were euthanized and single-cell suspensions prepared from the spleens were cultured for 48 h, then stimulated with purified protein HA1–2 (5 μg/ml). IFN-γ and IL-4 secretion was detected in triplicate with the ELISPOT assay.
