Fig. 2.

a Effect of medium composition on the rate. Reactions were performed in n-hexane:t-butanol:100 mM MOPS-NaOH using 50 mM VFA, 200 mM prenol at 40 °C, pH 6.0 for 8 h. Black system I (37.8:57.2:5.0 v/v/v), gray system II (53.4:43.4:3.2 v/v/v), striped system III (47.2:50.8:2.0 v/v/v), and white system IV (19.8:74.7:5.5 v/v/v). b Effect of VFA concentration on the rate. Reactions were performed at optimal medium composition for each enzyme, 200 mM prenol, 40 °C, pH 6.0 for 8 h. FaeA1 (black circle), FaeA2 (white circle), FaeB1 (black square), FaeB2 (white square), and MtFae1a (black rectangle) (rate is expressed as ×5 for FaeA1, FaeA2, and MtFae1a)