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. 2017 Jan 11;101(8):3213–3226. doi: 10.1007/s00253-017-8089-8

Table 1.

Biochemical characteristics of FAEs from M. thermophila

Enzyme ID Type Subfamily MW theoretical (kDa) MW observed (kDa) pI theoretical pI experimental Topt (°C) pHopt Source Reference FAE content (mg FAE/mg protein) Specific activity (U/mg FAE)
MFA PFA
FaeA1 JF826027.1a A SF5 29.2 29 5.9 ~5.5 45 6.5 C1 Kühnel et al. (2012) 0.337 15.952 (0.108) 14.425 (0.045)
FaeA2 JF826028.1a A SF5 31.8 36 5.5 ~5.2 40 7.5 C1 0.15 5.585 (0.091) 10.230 (0.009)
FaeB1 Sequence ID 71b B SF6 29.4 29 5.82 ND ND ND C1 Gusakov et al. (2011) 0.5 8.557 (0.290) 9.149 (0.101)
FaeB2 JF826029.1a B SF6 31.4 33 4.6 ~6.0 45 7.0 C1 Kühnel et al. (2012) 0.1 14.517 (0.917) 18.483 (0.939)
MtFae1a AEO62008.1a B SF6 31.4 39 4.6 ND 50 7.0 ATCC 42464 Topakas et al. (2012) 0.422 10.491 (0.864) 13.907 (0.663)

Numbers in the parentheses are the estimates of standard errors

ND not determined

aGenBank ID

bPatent ID