Table 2.
Apparent kinetic constants
| Enzyme | VFA | Prenol | ||||||
|---|---|---|---|---|---|---|---|---|
| v max (mol/g FAE L h) | K m (mM) | k cat (min−1 g FAE−1) | k cat/K m (M−1 min−1 g FAE−1) | v max (mol/g FAE L h) | K m (mM) | k cat (min−1 g FAE−1) | k cat/K m (M−1 min−1 g FAE−1) | |
| FaeA1 | 0.015 (0.002) | 54.5 (11.7) | 0.3698 | 6.783 | 0.073 (0.008) | 831.4 (173.0) | 1.752 | 2.107 |
| FaeA2 | 0.0052 (0.001) | 46.5 (18.1) | 0.156 | 3.358 | 0.025 (0.002) | 504.3 (92.8) | 0.735 | 1.457 |
| FaeB1 | 0.284 (0.020) | 30.6 (4.8) | 68.6 | 2245.1 | 0.270 (0.010) | 113.2 (18.6) | 65.2 | 492.2 |
| FaeB2 | 0.455 (0.054) | 31.6 (9.6) | 125.2 | 3956.7 | 0.516 (0.035) | 228.6 (53.1) | 141.7 | 619.9 |
| MtFae1a | 0.033 (0.005) | 81.2 (22.3) | 0.488 | 6.604 | 0.027 (0.001) | 186.9 (31.4) | 0.440 | 2.356 |
Numbers in the parentheses are the estimates of standard errors