Table 3.
Summary of optimal conditions and obtained parameters
| Enzyme | FaeA1 | FaeA2 | FaeB1 | FaeB2 | MtFae1a |
|---|---|---|---|---|---|
| Optimized conditionsa | |||||
| Water content (%) | 5.5 | 5.5 | 2.0 | 3.2 | 3.2 |
| VFA concentration (mM) | 80 | 50 | 50 | 60 | 100 |
| Prenol concentration (M) | 1 | 1 | 0.8 | 1 | 0.6 |
| Enzyme concentration (g FAE/L) | 0.1 | 0.1 | 0.1 | 0.02 | 0.2 |
| pH | 8 | 6 | 7 | 6 | 8 |
| Temperature (°C) | 55 | 45 | 40 | 30 | 30 |
| Time (h) | 24 | 48 | 24 | 24 | 24 |
| Obtained parameters | |||||
| PFA concentration (mM) | 32.9 (2.4) | 7.6 (0.3) | 24.1 (0.2) | 42.9 (0.1) | 42.7 (2.0) |
| PFA yield (%) | 41.1 (3.0) | 15.2 (0.5) | 48.1 (0.4) | 71.5 (0.2) | 42.7 (2.0) |
| Overall yield (%) | 93.9 (5.4) | 82.2 (0.5) | 83.1 (3.0) | 102.0 (4.7) | 63.2 (1.6) |
| Rate (mol PFA/g FAE L h) | 0.014 (0.001) | 0.0016 (0.0001) | 0.010 (0.000) | 0.089 (0.000) | 0.0089 (0.0004) |
| Initial rate (mol PFA/g FAE L h) | 0.053 (0.003) | 0.0057 (0.001) | 0.030 (0.002) | 0.182 (0.008) | 0.022 (0.004) |
| PFA/FA ratio | 0.778 (0.021) | 0.227 (0.011) | 1.378 (0.093) | 2.373 (0.362) | 1.700 (0.041) |
Numbers in the parentheses are the estimates of standard errors
aConcentrations are expressed as in total volume of reaction (500 μL)