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. 2017 Jan 9;13(2):226–243. doi: 10.1007/s12015-016-9713-1

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© The Author(s) 2017

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Characterization of EVs isolated from the conditioned medium (CM) of human MSCs and subjected to OptiPrep gradient separation. a Nanoparticle Tracking Analysis (NTA) profiles of particle size and distribution of EVs present in the concentrated CM of MSCs (cCM-EVs). cCM-EVs represent a extracellular vesicle population containing exosomes and microvesicles. Two peaks around 90–110 and 170–190 nm were detected. b Weight of the twelve different EV fractions generated by gradient separation of cCM-EVs. c NTA analysis of the percentage of EVs distributed in different fractions after gradient separation. Data reported are mean ± SEM of four different experiments