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Experimental flowchart. (a) ASCs were isolated from 3 donors and seeded in 4 different densities and 3 different culture conditions for 48 h. (b) Conditioned medium collection. ASCs were cultured with TNFα and IFNγ for 48 h. Thereafter, medium was refreshed with culture medium containing no TNFα and IFNγ. Following 24 h ASCs-conditioned medium samples were collected for analysis
