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. 2016 Dec 16;13(2):299–308. doi: 10.1007/s12015-016-9708-y

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — The presence of chondrogenic markers was demonstrated by immunofluorescence analysis. The human-induced pluripotent stem cells (hiPSC)-derived chondrocytes: DIRECT, TGF-β3, COND, TGF-β3+ COND did not reveal the presence of pluripotency markers: Nanog, OCT4, E-cadherin (a), but they did express desirable chondrogenic proteins: COMP, type II and IX collagen, aggreccan (AGG), SOX6 and SOX9 (b). The pluripotency and chondrogenic markers were quantified to better show the differences between particular differentiation protocols (c and d)