FIG 7 .

Characterization of KstR2 regulon mutants of M. smegmatis. Growth of ΔechA20, ΔfadE32, ΔipdF, and ΔipdAB M. smegmatis mutants on (A) 1.5 mM HIP or (C) 1 mM HIP plus 0.2% glycerol. Curves show WT (black), KstR2 regulon mutants (red), and corresponding complements (blue) and are the means from three biological replicates. (B) CoA metabolomes of mutants. The numbers correspond to CoASH (1), acetyl-CoA (2), HIEC-CoA (3), 5β-OH HIC-CoA (4), 5α-OH HIC-CoA (5), COCHEA-CoA (6), and unknown CoA thioester of 992 m/z (7). IS, p-coumaroyl-CoA internal standard. Lighter-shaded curves indicate the 768→261 transition (Fig. 4).