FIG 8 .

Cholesterol-dependent toxicity. (A) Growth of WT (black), ΔipdAB (red), and ΔipdAB::ipdAB (blue) M. tuberculosis grown on 7H9 medium containing 0.5 mM cholesterol and 0.2% glycerol. The data represent the average from biological triplicates. (B) The relative abundance of CoASH (768→261) was normalized to the internal standard (p-coumaroyl-CoA [914→407]) in KstR2 regulon mutants. *, P < 0.05 compared to the WT strain. Error bars represent standard deviations. The numbers of replicates were as follows: 5, 5, 5, and 3 for WT, ΔipdAB, ΔipdC, and ΔfadD3 ΔipdAB R. jostii RHA1, respectively; 2, 1, and 1 for WT, ΔipdAB, and ΔipdC M. tuberculosis (Mtb), respectively; and 4, 5, 1, 4, and 1 for WT, ΔipdAB, ΔechA20, ΔipdF, and ΔfadE32 M. smegmatis, respectively.