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. 2017 Mar 10;7(3):e539. doi: 10.1038/bcj.2017.16

Figure 2.

Figure 2

Knockdown of PI3K isoforms in MM. (a) MM1s cells and RPMI8226 were transduced with lentivirus targeted to PI3Kδ and PI3Kγ or control shRNA for 72 h. RNA was extracted and analysed for either PI3Kδ or PI3Kγ messenger RNA expression by real-time PCR. GAPDH was used to normalise RNA values. (b) MM1s cells and RPMI8226 were transduced with lentivirus targeted to PI3Kδ and PI3Kγ or control shRNA for 5 days. Cells were then assayed for viability by Cell Titre Glo assay. (c) MM1s cells and RPMI8226 were transduced with lentivirus targeted to PI3Kδ and PI3Kγ or control shRNA for 5 days. Cells were then assessed for apoptosis by PI/Annexin V staining.