Figure 6.

Inhibition of PI3Kδ/γ in vivo leads to reduced engraftment and increased survival. (a, b) U266-luc were transduced with lentivirus targeted to PI3Kδ and PI3Kγ or PI3Kδ/γ or control shRNA for 96 h. About 0.5 × 10⁶ cells lentiviral transduced U266-luc were subsequently were i.v. injected into NSG mice via the tail vein. (a) Bioluminescence (BLI) was used to track the U266-luc cells in vivo. (b) Survival of the NSG mice is represented by a Kaplan–Meier plot. (c, d) About 0.5x10⁶ cells were i.v. injected into NSG mice. At day 5 after i.v. injection mice were treated i.p. with IP1-145 (15 mg/kg per day) or vehicle control. (c) BLI was used to track the U266-luc cells in vivo. (d) Survival of the NSG mice is represented by a Kaplan–Meier plot.