Figure 1.

Langerin is exclusively expressed by human LCs. (a) Staining of a section of steady-state human skin for langerin (blue), CD1a (green), CD14 (red), and Hoechst (yellow), and analysis by fluorescence microscopy. (b) Gating strategy for FACS-sorted LCs, CD14⁺, CD1a⁺, and double-negative dermal DCs. (c) Langerin mRNA is exclusively expressed in primary, FACS-sorted LCs and not by the other skin DC subsets. N = 3; each experiment contained sorted cells from at least five skin donors. mRNA values are normalized to GAPDH levels.