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. 2015 Oct 12;14(4):360–370. doi: 10.1038/cmi.2015.87

Figure 5.

Figure 5

Langerin routes to less degradative endosomal compartments in LCs after antigenic pulse compared to dectin-1 A+B. The remaining fluorescence of anti-langerin (a) and anti-dectin-1 antibodies (b) in a pulse–chase in the presence or absence of pI:C. C+D. Pulse–chase of anti-langerin and anti-dectin-1 antibody degradation in LCs by western blotting using an anti-mouse Fc peroxidase-labeled antibody. Staining is shown in (c), and band intensity quantification in (d). (e) Co-localization of langerin and dectin-1 with the intracellular compartments EEA-1 and LAMP-1 after receptor binding and internalization at the cell surface. (f) Three-color co-localization score of langerin, dectin-1 and EEA-1, or LAMP-1 in resting conditions (left panel) or after receptor triggering at the cell surface (right panel) measured by imaging flow cytometry (representative images are shown in Supplementary Figure S4). (g) Randomly selected LCs representing the data depicted in e (15-min time point).