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. 2017 Apr 5;7:46065. doi: 10.1038/srep46065

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Copyright © 2017, The Author(s)

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Immunoblot analysis was performed with a specific antibody against phosphorylated NF-κB and phosphorylated IκB-α. Data were normalized against the density of β-action by TotalLab TL100 v2006 software (A). Immunohistochemical staining was performed with a specific antibody against F4/80. A few F4/80-positive cells were observed in the peritubular areas (arrow) and interstitial foci (arrowhead) of ALA and ALA + sham kidneys (B) and MCP-1 (C). Scale bar; 100 μm. ALA; only ALA treated group, ALA + Sham; ALA treated and no ureteral ligated group, UUO; no ALA treated, but ureteral ligated group, UUO + ALA; ALA treated and ureteral ligated group. Values are expressed as means ± SE (*P < 0.05).