Skip to main content
. 2017 Apr 5;7:46029. doi: 10.1038/srep46029

Figure 1. The stage of the peripheral B cell maturation block in Xid mice shifts upstream upon removal of CD4 T cell help.

Figure 1

Ex-vivo splenic cells from mice of indicated genotypes were stained for B220, CD93, CD23, CD21/35 and IgM to estimate (as shown in Fig. S1), (A) numbers of total B cells and of B cell subsets in WT and Xid mice (n = 13), (B) numbers of total B cells and (C,D) of B cell subsets in Xid versus Xid + TCRbeta-null littermate and Xid versus Xid + CD40-null littermate mice, (n = 9). (E) Sera from 4–8 week old littermate mice of various genotypes as shown were analyzed for IgM and IgG levels (n ≥ 5). (F,G) Ex-vivo splenic cells from mice of indicated genotypes were stained for B220, CD93, CD23, CD21/35 and IgM to estimate numbers of total B cells (F) and of B cell subsets (G) in Xid versus Xid + MHCII-null littermate mice (as shown in Fig. S1; n = 6). *p < 0.05; **p < 0.005.