Figure 2.
Formation of CS-ZN-NIMs using a single water-in-oil emulsion (W/O). Briefly, CS/DNA NPs were formed via ionic gelation as described. The formed NPs were added to a 90% aqueous ethanol solution of ZN at varying concentrations (5–25% w/v). The ZN-CS/DNA NPs suspension was then added to the continuous phase (corn oil) under constant stirring to form the single W/O emulsion. Solvent evaporation results in ZN microsphere solidification, encapsulating the CS/DNA NPs to form CS-ZN-NIMs. The resulting particles were separated from the continuous phase, washed to remove oil, and collected.