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. Author manuscript; available in PMC: 2018 Mar 10.
Published in final edited form as: J Control Release. 2017 Jan 31;249:150–161. doi: 10.1016/j.jconrel.2017.01.035

Figure 2.

Figure 2

Formation of CS-ZN-NIMs using a single water-in-oil emulsion (W/O). Briefly, CS/DNA NPs were formed via ionic gelation as described. The formed NPs were added to a 90% aqueous ethanol solution of ZN at varying concentrations (5–25% w/v). The ZN-CS/DNA NPs suspension was then added to the continuous phase (corn oil) under constant stirring to form the single W/O emulsion. Solvent evaporation results in ZN microsphere solidification, encapsulating the CS/DNA NPs to form CS-ZN-NIMs. The resulting particles were separated from the continuous phase, washed to remove oil, and collected.