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. Author manuscript; available in PMC: 2018 Mar 10.
Published in final edited form as: J Control Release. 2017 Jan 31;249:150–161. doi: 10.1016/j.jconrel.2017.01.035

Figure 8.

Figure 8

Anti-GFP IgA titers following a single oral delivery of CS-ZN-NIMs. CS-ZN-NIM amounts equivalent to 35 and 100 μg of pEGFP-LUC were delivered to 6-week old male BALB/c mice via oral gavage. Three weeks after gavage, fecal samples were collected, diluted 1:20 w/v in PBS and anti-GFP IgA antibody titers were evaluated using an ELISA assay. Fecal samples from mice immunized with non-GFP antigen was used as a negative control. Endpoint titers were determined using the optical densities measured for the negative control as a cutoff for endpoint dilutions. ( *** p ≤ 0.001, ** p ≤ 0.01)