Figure 5. Bacterial growth curves and dose-dependent growth inhibition of bacteria treated with histone H5 (broth microdilution assay).

(A) MSSA and (B) MRSA strains were treated with increasing concentrations of histone H5 in sterile H₂O, pH 7.4: 0.5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 8 μg/ml, and kanamycin 1 mg/ml as a positive control for inhibition. The untreated control cells were incubated with sterile H₂O, pH 7.4 as a negative control for inhibition. Results are representative of three independent trials, each in triplicate. Statistical analysis was done by Student’s T-Test, (*) indicates P ≤ 0.01 compared with the untreated control cells for MSSA bacterial growth; (+) indicates P ≤ 0.0001 compared with the untreated control cells for MRSA bacterial growth.