Table 1. Densitometry and LC/MS/MS proteomics analysis results of purified chicken histones.
| Bandsa | Identified proteinsb | Number of unique peptidesb | UniProt IDb | % of total histone sample (±SD)c | Coverage (%)b |
|---|---|---|---|---|---|
| Band 1 | Histone H1.11L | 41 | P08287 | 3.7 ± 0.6 | 49 |
| Histone H1.01 | 32 | P08284 | 47 | ||
| Histone H1.03 | 36 | P08285 | 44 | ||
| Band 2 | Histone H1.11R | 26 | P08288 | 6 ± 1 | 51 |
| Histone H1.01 | 25 | P08284 | 46 | ||
| Histone H1.11L | 23 | P08287 | 43 | ||
| Band 3 | Histone H5 | 25 | P02259 | 18 ± 1 | 50 |
| Band 4 | Histone H3.2 | 19 | P84229 | 24 ± 1 | 62 |
| Band 5 | Histone H2B 1/2/3/4/6Histone H2B 8Histone H2B 7 | 27 | P0C1H3 | 19 ± 1 | 75 |
| 26 | Q9PSW9 | 75 | |||
| 25 | P0C1H5 | 69 | |||
| Band 6 | Histone H2A (III, IV) | 18 | P35062, P02263 | 15 ± 1 | 72 |
| Histone H2A.V | 6 | P02272 | 54 | ||
| Histone H2A (fragment) | 3 | F1P5G5 | 16 | ||
| Band 7 | Histone H4 | 29 | P62801 | 14.6 ± 0.4 | 73 |
aBands are depicted in Fig 1. bData obtained from Scaffold, Proteome Software Inc. cResults obtained from densitometry analysis using ImageJ densitometry software version 1.6; SD, standard deviation.