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. 2017 Apr 5;7:45962. doi: 10.1038/srep45962

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(a) Western blot of 4 dpf wild-type and zGc3 morphant heads with anti-zGc3 antibody. A band of approximately 120 kDa was detected in wild-type and control morpholino-injected embryos, but absent in zGc3-ATG and splicing morpholino-injected embryos. Histone H3 served as loading control. (b) Western blot of 4 dpf wild-type and zGc3 morphant heads with anti-Pde6c antibody. A band of approximately 100 kDa was detected in wild-type, and control morpholino-injected embryos, but markedly reduced in zGc3-ATG and splicing morpholino-injected embryos. Histone H3 served as loading control.