Fig. 1.

Reduced secretion levels of AmyE, AmyL and BPN’ in rasP mutant cells. ΔrasP mutant cells and wild-type (wt) or ΔtepA mutant control cells expressing AmyE, AmyL or BPNʹ were grown for 16 h in MBU medium. Next, cells and growth media were separated by centrifugation. Proteins in growth medium fractions were precipitated with TCA and analyzed by LDS-PAGE. Protein bands were visualized with the SimplyBlue SafeStain. Molecular weights of marker proteins are indicated (in kDa) on the left side of each gel segment. Secreted amounts of AmyE, AmyL and BPNʹ in the growth medium fractions from three independent cultures were assessed by ImageJ analysis of the gels, and the ratios of each of these proteins in the medium fractions of the ΔrasP or ΔtepA strains relative to the wt strain are indicated below each gel segment together with the standard deviation