Fig. 5.

Improved production of Properase and AmyAc upon overexpression of rasP. a Growth (left panel) and extracellular Properase activity (right panel) of cells cultured in MBU medium. Measurements on cells that overexpress rasP from the strong PspoVG promoter are indicated with black lines and measurements on wt cells are indicated with grey lines. b Growth (left panel) and extracellular AmyAc activity (right panel) of cells that overexpress rasP from the PspoVG promoter (black lines) or wt cells (grey lines) cultured in MBU medium. c Growth (left panel) and extracellular AmyAc activity (right panel) of cells that overexpress rasP from the PspoVG promoter (black lines) or wt cells (grey lines) cultured in 5SM12 medium. All plots in a–c show average values from three independent experiments, and the error bars represent the standard deviations of the respective measurements