Table 1.
Bacterial strains used in this study
| Relevant genotypes and phenotypes | Source or reference | |
|---|---|---|
| B. subtilis strain | ||
| Δupp | degUHy32, amyE::xylRPxylAcomK-ermC, Δupp::neoR, NeoR | This study |
| AmyL | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyL catR Tbpnʹ; EmR; CmR | This study |
| AmyE | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyE catR Tbpnʹ; EmR; CmR | This study |
| BPNʹ | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-bpnʹ catR Tbpnʹ; EmR; CmR | This study |
| ΔrasP | degUHy32, amyE::xylRPxylAcomK-ermC, Δupp::neoR, ΔrasP::upp-phleoR-cI EmR; PhleoR; NeoS | This study |
| AmyL ΔrasP | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyL catR, Δupp::neoR, ΔrasP::upp-phleoR-cI catR; EmR; PhleoR; NeoS CmR | This study |
| AmyE ΔrasP | degUHy32, ∆scoC, amyE::xylRPxylAcomK-ermC, ∆opp, aprE::PaprE-amyE catR, Δupp::neoR, ΔrasP::upp-phleoR-cI catR; EmR; PhleoR; NeoS CmR | This study |
| BPNʹ ΔrasP | degUHy32, ∆scoC, amyE::xylRPxylAcomK-ermC, ∆opp, aprE::PaprE-bpn’ catR; Δupp::neoR, ΔrasP::upp-phleoR-cI EmR; PhleoR; NeoS CmR | This study |
| ΔtepA | degUHy32, amyE::xylRPxylAcomK-ermC, Δupp::neoR, ΔtepA::upp-phleoR-cI EmR; PhleoR; NeoS | This study |
| AmyL ΔtepA | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyL catR, Δupp::neoR, ΔtepA::upp-phleoR-cI catR; EmR; PhleoR; NeoS CmR | This study |
| AmyE ΔtepA | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyE catR, Δupp::neoR, ΔtepA::upp-phleoR-cI catR; EmR; PhleoR; NeoS CmR | This study |
| BPNʹ ΔtepA | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-bpnʹ catR; Δupp::neoR, ΔtepA::upp-phleoR-cI EmR; PhleoR; NeoS CmR | This study |
| Properase | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-properase catR Tbpnʹ; EmR; CmR | This study |
| Properase RasP | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-properase catR Tbpnʹ, spoIIIAH::PspoVG-rasP; EmR; CmR | This study |
| AmyAc | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyAc catR Tbpnʹ; EmR; CmR | This study |
| AmyAc RasP | degUHy32, amyE::xylRPxylAcomK-ermC, aprE::PaprE-amyAc catR Tbpnʹ, spoIIIAH::PspoVG-rasP; EmR; CmR | This study |
| Plasmids | ||
| pHT315-Pspac | Multicopy shuttle vector replicating in E. coli and B. subtilis contains IPTG-inducible Pspac; AmpR, EmR | Genencor/dupont [44] |
| pHTK315 | pHT315-Pspac derivative, EmR is replaced by KmR; AmpR, KmR | This study |
| pHT315K::rasP | pHT315K-Pspac derivative, contains IPTG-inducible rasP; AmpR, KmR | This study |
Tbpnʹ terminator structure of B. amyloliquefaciens bpnʹ; Em R erythromycin resistant; Phleo R phleomycin resistant; Neo R neomycin resistant; Neo S neomycin sensitive; Cm R chloramphenicol resistant; Amp R ampicillin resistant; Km R kanamycin resistant