Table 2.
Primers used in this study
| Primer name | 5′ → 3′ nucleotide sequence |
|---|---|
| CI2.rev | CTTCAACGCTAACTTTGAG |
| rasP.P0 | GCTCTTCAAGGCGAACAGG |
| rasP.P1 | CGCCTCATCATTACGGCATC |
| rasP.P2 | CGACCTGCAGGCATGCAAGCTACCACCTTATGTGAGTATTGAATTGAC |
| rasP.P3 | CGAGCTCGAATTCACTGGCCGTCGGGATACGTCAATTCAATACTCACATAAGGTGGTACGAAAAGTAAATCAATCAGAGGTGC |
| rasP.P4 | GATCGTACGGCGCAACG |
| tepA.P0 | CGCACGGGCACGATG |
| tepA.P1 | CTGTCCGTTCCAGTGTACGG |
| tepA.P2 | CGACCTGCAGGCATGCAAGCTCTCGCTTTCATCCTTTCCG |
| tepA.P3 | CGAGCTCGAATTCACTGGCCGTCGCAAAGAGAAACTCGGAAAGGATGAAAGCGAGTTCTTTATACCGTGATGCCTCAG |
| tepA.P4 | GGTCTGTCATTCAATTTAGACTCCAG |
| pHT315CPEC.fw | CTATGAGTCGCTTTTTTAAATTTGGAAAGTTAC |
| pHT315CPEC.rev | CACTGTTTTTAGTCTGTTTCAAAACAGTAG |
| kanaCPEC.fw | CTACTGTTTTGAAACAGACTAAAAACAGTGGCTCCGTCGATACTATGTTATACG |
| kanaCPEC.rev | TAACTTTCCAAATTTAAAAAAGCGACTCATAGGCTTTTTAGACATCTAAATCTAGGTAC |
| pHT315rasP.fw | GTGAGTATTGAATTGACGTATCCCCGCCCGGGTACCGAGCTCTTAATTG |
| pHT315rasP.rev | GCGAAGAAATGAGACAAAGCTTGACGGCTTGGCGTAATCATGGTCATAGC TG |
| rasP.fw | CGGGGATACGTCAATTCAATACTCAC |
| rasP.rev | CGTCAAGCTTTGTCTCATTTCTTCGC |