Fig. 2.

Radiation promotes the TUG1 and HMGB1 expression in bladder cancer cell lines. a qRT-PCR analysis was conducted to detect the TUG1 expression in bladder cancer cell lines SW780, HT1376, BIU87 and T24 and normal bladder epithelial cell line HCV-29. b The protein level of HMGB1 was determined by western blot in SW780 and BIU87 cells. c TUG1 expression was detected in SW780 and BIU87 cells every 3 h after 0 or 2 Gy of ionizing radiation treatment. d TUG1 expression was measured in SW780 and BIU87 cells after different doses (0, 2, 4, 6 Gy) of ionizing radiation treatment for 24 h. e The HMGB1 protein level was upregulated after SW780 and BIU87 cells treated with 2 Gy of ionizing radiation for 24 h. *P < 0.05 vs. NC