Fig. 3.

TUG1 silencing enhances radiosensitivity of bladder cancer cell lines. a qRT-PCR analysis of the relative TUG1 expression in SW780 and BIU87 cells transfected withTUG1-specific si-RNAs or control si-RNA. SW780 and BIU87 cells were transfected with si-NC or si-TUG1 and cultured for 24 h followed by ionizing radiation. b The cell viability of transfected SW780 and BIU87 cells at 0, 24, 48, 72 and 96 h afterionizing radiation. c and d Cell apoptosis was determined in transfected SW780 and BIU87 cells at 24 h after 2 Gy radiotherapy. e Transfected SW780 and BIU87 cells were subjected to 0, 2, 4, 6 and 8 Gy of irradiation. After 2 weeks, the colony survival fractions were measured. *P < 0.05 vs. si-NC