Table 2.
Real-time PCR verification of differentially expressed genes in mutant strain ∆22915
| Gene locusa | Description of genes | Functionb | Fold changes (2−∆∆Ct) ±SD |
|---|---|---|---|
| BAB_RS24460 | Extracellular ligand-binding receptor | Amino acid transport and metabolism | 39.08 ± 4.25 |
| BAB_RS17405 | Dihydropyrimidinase | Nucleotide transport and metabolism | 20.50 ± 2.27 |
| BAB_RS30485 | Extracellular solute-binding protein family 1 | Amino acid transport and metabolism | 15.35 ± 1.80 |
| BAB_RS17430 | Oxidoreductase | Amino acid transport and metabolism | 12.67 ± 1.25 |
| BAB_RS31735 | Amidohydrolase | Function unknown | 3.63 ± 0.27 |
| BAB_RS27765 | Fumarylacetoacetate (Faa) hydrolase | Function unknown | 2.80 ± 0.22 |
| BAB_RS30280 | Quinone oxidoreductase | Energy production and conversion | 1.82 ± 0.20 |
| BAB_RS30270 | Abc transporter permease protein | Amino acid transport and metabolism | 1.70 ± 0.24 |
| BAB_RS26970 | Flagellar basal-body rod protein | Cell motility | 1.62 ± 0.31 |
| BAB_RS18915 | Gene transfer agent | Function unknown | 1.55 ± 0.22 |
| BAB_RS30285 | Transcriptional regulator, GntR family | Transcription | 1.52 ± 0.26 |
| BAB_RS30275 | Extracellular ligand-binding receptor | Amino acid transport and metabolism | 1.37 ± 0.14 |
| BAB_RS28745 | Abc transporter permease protein | Amino acid transport and metabolism | 1.37 ± 0.35 |
| BAB_RS 28215 | Transposase | Replication, recombination and repair | 1.25 ± 0.23 |
| BAB_RS27910 | Transcriptional regulator | Transcription | 0.81 ± 0.19 |
| BAB_RS22920 | Auxin efflux carrier | Function unknown | 0.76 ± 0.11 |
Genes with over twofold changes levels and high probability were further validated with qRT-PCR.
aBased on B. abortus S2308 genome (GenBank Code: NC_007618.1 and NC_007624.1).
bThe functional categories of protein were predicted by searching through EggNOG database [33] with BLASTP.