Fig. 1.

Procyanidins suppressed MSU-induced NLRP3 inflammasome activation in RAW macrophages. a The cells were stimulated by LPS (1 μg/ml) for 6 h and then stimulated with MSU crystals for another 6 h. Procyanidins were added 20 min before LPS administration. Western blot samples were prepared from the supernatant (n = 4). b The cell extracts were collected at 12 h following LPS treatment from Raw 264.7 cells; procyanidins were added 20 min before LPS (n = 4). c The cells were stimulated by LPS (1 μg/ml) for 6 h and then stimulated with MSU crystals for another 6 h. Procyanidins (10 μM) were added 20 min before LPS. The supernatant (SN) and cell lysis fractions (Input) of Raw 264.7 cells were collected respectively (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001 vs. naive; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. the MSU-treated group