Fig. 2.

Procyanidins suppressed MSU-induced ROS production in macrophages. a The levels of ROS were assessed by calculating the ratio of positive-staining cells among 10,000 cells using flow cytometry. Raw 264.7 cells were primed with procyanidins (10 μM) for 20 min and then stimulated by MSU crystals for 3 h. b MTT assay: Raw 264.7 cells were treated with various doses of procyanidins (1, 5, and 10 μM) for 24 h