Fig. 4.

Procyanidins suppressed NLRP3 inflammasome activation in ankle periarticular tissue. Mice were treated with various doses of procyanidins (15, 30, and 60 mg/kg, PO) 20 min before the injection of MSU crystals (0.5 mg/10 μL). Western blot samples were collected 24 h after MSU injection (n = 4). Expression of pro- and cleaved IL-1β (a), pro- and cleaved caspase-1 (b), and NLRP3 (c) in ankle periarticular tissue are shown. *p < 0.05, **p < 0.01, ***p < 0.001 vs. normal; ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. the MSU-treated group