. 2017 Apr 4;17:83. doi: 10.1186/s12866-017-0994-6

Table 1.

Oligonucleotide sequence for the amplification of mecA, orfX and SCCmec types

S/N	Primer	Oligonucleotide sequence 5’to 3′	Product size	Annealing temperature	Reference
1	mecAF mecAR	ACTGCTATCCACCCTCAAAC	163 bp	57 °C/120 s	Noto. [27]; Mehrotra et al. [28]
1	mecAF mecAR	CTGGTGAAGTTGTAATCTG	163 bp	57 °C/120 s	Noto. [27]; Mehrotra et al. [28]
2	Nuc-F	GCG ATT GAT GG TGA TAC GGT T	276 bp	55 °C/120 s	Saiful et al. [29]
2	Nuc-R	AGC CAA CGG TTG ACG AAC TAA AGC	276 bp	55 °C/120 s	Saiful et al. [29]
3	OrfX F	GAG AAA TAT TGG AAG CAA GCC	326 bp	54.6 °C/60s	Noto. [27]
3	OrfX R	CGC ATA ATC TTA AAT GCT CTG	326 bp	54.6 °C/60s	Noto. [27]
4	SCCmecIIIFSCCmecIIIR	TTC TCA TTG ATG CTG AAG CC	280 bp	55 °C /60 s	Zhang et al. [30]
4	SCCmecIIIFSCCmecIIIR	GTG TAA TTT CTT TTG AAA GAT ATG G	280 bp	55 °C /60 s	Zhang et al. [30]
5	mecA F	5′-ACTGCTATCCACCCTCAAAC-3′	533 bp	55 °C/120 s	Saiful et al. [29]
5	mecA R	5′-CTGGTGAAGTTGTAATCTGG-3′	533 bp	55 °C/120 s	Saiful et al. [29]