Table 1.
LDR primers and microspheres used to detect G6PD polymorphisms
Polymorphisma | LDR primersb | Microsc |
---|---|---|
A- | 5′-tacaacatctcattaacatatacaAGGCGGGAACGGGCATAGCCCAC-3′ | 37 |
202G > A | 5′-caaacaaacattcaaatatcaatcAGGCGGGAACGGGCATAGCCCAT-3′ | 22 |
rs1050825 | common5′-Phos-GATGAAGGTGTTTTCGGGCAGAA-Biotin-3′ | |
A | 5′-cttaacatttaacttctataacacTGTGACCCCAGGTGGAGGGCATT-3′ | 30 |
3769A > G | 5′-tacattcaacactcttaaatcaaaTGTGACCCCAGGTGGAGGGCATC-3′ | 26 |
rs1050829 | common5′-Phos-CATGTGGCTGTTGAGGCGCTGGT-Biotin-3′ | |
Mediterranean | 5′-aatttcttctctttctttcacaatTCTGGTCCTCACGGAACAGGGAGG-3′ | 14 |
188C > T | 5′-cacttaattcattctaaatctatcTCTGGTCCTCACGGAACAGGGAGA-3′ | 28 |
rs5030868 | common5′-Phos-AGATGTGGTTGGACAGCCGGTCA-Biotin-3′ | |
Silent | 5′-cataatcaatttcaactttctactAAGACGTCCAGGATGAGGCGCTCG-3′ | 12 |
1311C < T | 5′-acttatttcttcactactatatcaAAGACGTCCAGGATGAGGCGCTCA-3′ | 34 |
rs2230037 | common5′-Phos-TAGGCGTCAGGGAGCTTCACGTT-Biotin-3′ |
aEach polymorphic site includes information regarding legacy allele names, cDNA nucleotide change and RefSNP accession ID (rs)
bLDR-FMA probes are based on positive-strand sequence. Polymorphic nucleotides are underlined and bold. Lower case letters represent 24 bp tags complementary to unique microsphere anti-tags
cUnique microsphere sets designed by Luminex Corp