Table 1. Partial list of the differentially abundant proteins identified in FC2 exposed to N-starvation as a function of time.
| Sl. No. | Acc_number | Entry_name | Gene ID | Uniq pepa | 40/0 h | 88/0 h | 120/0 h |
|---|---|---|---|---|---|---|---|
| Nitrogen assimilation, amino acid biosynthesis and Protein degradation | |||||||
| 1. | E1Z7W6 | Ferredoxin-dependent glutamate synthase, chloroplastic | CHLNCDRAFT_142154 | 3 | 1.167 ± 0.28 | 1.750 ± 0.97 | 1.716 ± 0.63 |
| 2. | E1Z4T9 | Aspartate aminotransferase | CHLNCDRAFT_137913 | 1 | 1.443 ± 0.07 | 2.082 ± 0.23 | 2.289 ± 0.41 |
| 3. | A0A087SJX6 | Argininosuccinate synthase | F751_3668 | 1 | NS | 2.314 ± 0.32 | 1.891 ± 0.26 |
| 4. | E1ZIW5 | Arginine biosynthesis bifunctional protein ArgJ, chloroplastic | CHLNCDRAFT_135738 | 1.407 ± 0.55 | NS | 2.877 ± 2.32 | |
| 5. | E1ZF33 | Putative uncharacterized protein (arginine biosynthesis) | CHLNCDRAFT_52216 | 1 | 1.563 ± 0.96 | 1.797 ± 1.13 | 1.446 ± 0.64 |
| 6. | E1ZEF2 | Cysteine synthase | CHLNCDRAFT_145435 | 3 | 1.317 ± 0.24 | 1.804 ± 0.03 | 2.065 ± 0.02 |
| 7. | E1ZP71 | 3-isopropylmalate dehydrogenase | CHLNCDRAFT_56307 | 1 | 1.190 ± 0.52 | 1.626 ± 0.75 | 1.485 ± 0.97 |
| 8. | E1Z6E6 | Peptidyl-prolyl cis-trans isomerase | CHLNCDRAFT_29941 | 1 | 1.224 ± 0.47 | 2.051 ± 0.45 | 2.309 ± 0.71 |
| 9. | E1Z5I7 | Ubiquitin-60S ribosomal protein L40-2 | CHLNCDRAFT_48528 | 4 | 1.038 ± 0.39 | 1.930 ± 0.55 | 2.337 ± 0.70 |
| 10. | E1ZCR2 | Proteasome subunit alpha type | CHLNCDRAFT_48764 | 1 | 0.987 ± 0.20 | 1.478 ± 0.38 | 1.741 ± 0.28 |
| 11. | A0A087SEY5 | Proteasome subunit alpha type-4 | F751_5131 | 1 | 1.035 ± 0.24 | 1.693 ± 0.13 | 2.566 ± 0.12 |
| 12. | E1Z575 | Putative uncharacterized protein (Cytosol aminopeptidase) | CHLNCDRAFT_33905 | 1 | 1.493 ± 0.60 | 1.956 ± 0.50 | 2.047 ± 0.19 |
| 13. | E1ZMK0 | Putative uncharacterized protein (metallo peptidase) | CHLNCDRAFT_137495 | 1 | 0.335 ± 0.07 | 0.835 ± 0.29 | 2.149 ± 1.37 |
| 14. | E1ZN67 | Proteasome subunit alpha type | CHLNCDRAFT_26444 | 3 | 1.532 ± 0.02 | 1.666 ± 0.14 | 2.599 ± 0.33 |
| 15. | E1ZQQ9 | Proteasome subunit alpha type | CHLNCDRAFT_27583 | 2 | 0.864 ± 0.49 | 1.676 ± 0.58 | 2.030 ± 0.85 |
| 16. | E1ZPZ3 | Dihydroxy-acid dehydratase | CHLNCDRAFT_58991 | 2 | 1.419 ± 0.57 | 2.159 ± 1.25 | 2.117 ± 0.78 |
| Photosynthesis | |||||||
| 17 | E1Z6S6 | Putative uncharacterized protein (photosystem II assembly) | CHLNCDRAFT_140330 | 1 | 1.451 ± 0.19 | 2.346 ± 0.38 | 2.684 ± 0.55 |
| 18 | E1ZBP9 | Ferredoxin–NADP reductase | CHLNCDRAFT_35035 | 3 | 1.312 ± 0.12 | 1.853 ± 0.19 | 2.121 ± 0.17 |
| 19 | E1ZQR2 | Putative uncharacterized protein | CHLNCDRAFT_32868 | 1 | 1.139 ± 0.11 | 2.508 ± 0.76 | 3.099 ± 0.70 |
| 20 | E1ZPZ7 | Putative uncharacterized protein | CHLNCDRAFT_139312 | 2 | 1.004 ± 0.17 | 1.781 ± 0.57 | 1.541 ± 0.11 |
| 21 | E1ZRQ7 | Porphobilinogen deaminase, chloroplastic | CHLNCDRAFT_33052 | 2 | 0.775 ± 0.23 | 1.153 ± 0.30 | 0.962 ± 0.14 |
| Carbon metabolism | |||||||
| 22 | F2YGL1 | Large subunit of Rubisco | rbcL | 3 | 1.181 ± 0.40 | 1.422 ± 0.61 | 1.589 ± 0.48 |
| 23 | A0A087SAW7 | Ribulose bisphosphate carboxylase small chain | F751_5580 | 1 | 1.439 ± 0.19 | 1.961 ± 0.33 | 2.192 ± 0.12 |
| 24 | E1ZRS4 | Malate dehydrogenase(b),(c),(d) | CHLNCDRAFT_59812 | 3 | 1.539 ± 0.28 | 2.308 ± 0.47 | 3.312 ± 0.17 |
| 25 | E1ZT20 | Glyceraldehyde-3-phosphate dehydrogenase | CHLNCDRAFT_49269 | 2 | 1.073 ± 0.31 | 1.604 ± 0.24 | 1.159 ± 0.25 |
| 26 | E1ZQQ5 | Fructose-bisphosphate aldolase | CHLNCDRAFT_37179 | 2 | 1.643 ± 0.86 | 1.647 ± 0.73 | 3.292 ± 2.08 |
| 27 | E1ZF27 | Phosphoribulokinase(c),(d) | CHLNCDRAFT_31168 | 3 | 1.280 ± 0.05 | 1.363 ± 0.22 | 1.137 ± 0.17 |
| 28 | A0A087SU66 | Pyruvate kinase | F751_1646 | 1.418 ± 0.18 | 2.289 ± 0.01 | 1.980 ± 0.14 | |
| 29 | E1Z2U6 | Phosphoenolpyruvate carboxykinase [ATP] 1 | CHLNCDRAFT_56532 | 2 | 1.147 ± 0.66 | 2.179 ± 1.05 | 2.947 ± 0.71 |
| 30 | E1Z5A0 | Phosphoglycerate kinase | CHLNCDRAFT_29609 | 2 | 1.596 ± 0.25 | 2.381 ± 0.65 | 3.144 ± 0.71 |
| 31 | E1Z6L2 | Sedoheptulose-1,7-bisphosphatase(b),(d) | CHLNCDRAFT_19601 | 2 | 0.437 ± 0.19 | 0.638 ± 0.15 | 0.723 ± 0.09 |
| 32 | E1Z7C4 | Putative uncharacterized protein (Ribose-5-phosphate isomerase)(b),(d) | CHLNCDRAFT_34303 | 1 | 1.164 ± 0.11 | 2.164 ± 0.77 | 2.584 ± 0.15 |
| 33 | E1Z7S4 | Ribulose-phosphate 3-epimerase | CHLNCDRAFT_56033 | 1 | 0.800 ± 0.02 | 1.015 ± 0.16 | 1.642 ± 0.33 |
| 34 | E1ZKS0 | Enolase | CHLNCDRAFT_136652 | 2 | 1.688 ± 0.12 | 2.244 ± 0.81 | 1.723 ± 1.50 |
| 35 | E1Z1Z7 | Putative uncharacterized protein | CHLNCDRAFT_29144 | 1.022 ± 0.44 | 1.433 ± 0.03 | 3.032 ± 0.67 | |
| 36 | E1ZKB3 | Triosephosphate isomerase(b),(c),(d) | CHLNCDRAFT_36334 | 2 | 1.223 ± 0.69 | 2.184 ± 1.52 | 2.232 ± 0.96 |
| 37 | E1ZRS1 | Reversibly glycosylated protein(b),(d) | CHLNCDRAFT_56392 | 3 | 1.736 ± 0.11 | 3.077 ± 0.15 | 3.329 ± 0.20 |
| Fatty acid metabolism | |||||||
| 38 | E1Z5W8 | Acyl carrier protein | CHLNCDRAFT_29840 | 1 | 0.757 ± 0.28 | 1.499 ± 0.75 | 1.618 ± 0.42 |
| 39 | E1Z2Y2 | Putative uncharacterized protein (enoyl-[acyl-carrier protein] reductase I) | CHLNCDRAFT_59537 | 1 | NS | NS | 2.319 ± 1.61 |
| 40 | E1Z8J0 | Putative uncharacterized protein (hydro-lyase activity) | CHLNCDRAFT_34566 | 1 | 0.964 ± 0.09 | 1.472 ± 0.27 | 2.131 ± 0.32 |
| 41 | E1ZIL0 | Putative uncharacterized protein (Acyl-CoA dehydrogenase) | CHLNCDRAFT_24792 | 1 | 2.195 ± 0.49 | 1.779 ± 0.02 | 2.151 ± 0.09 |
| 42 | E1ZES7 | Putative uncharacterized protein (phosphotransferase) | CHLNCDRAFT_57872 | 1 | 1.382 ± 0.63 | 2.135 ± 2.29 | 2.539 ± 2.60 |
| Stress responsive | |||||||
| 43 | E1Z580 | Superoxide dismutase(d) | CHLNCDRAFT_33910 | 4 | 1.000 ± 0.57 | 1.928 ± 0.89 | 2.152 ± 1.18 |
| 44 | E1ZG17 | Putative uncharacterized protein (Fragment) (peroxidin activity) | CHLNCDRAFT_23497 | 2 | 1.327 ± 0.36 | 2.203 ± 0.82 | 2.108 ± 0.73 |
| 45 | E1ZCK9 | Putative uncharacterized protein (Chloroplastic lipocalin) | CHLNCDRAFT_145104 | 2 | 1.398 ± 0.60 | 1.773 ± 0.41 | 2.209 ± 0.30 |
| 46 | A0A087SDD7 | Octanoyltransferase | F751_0955 | 1 | 1.379 ± 0.44 | 1.963 ± 0.87 | 2.809 ± 0.97 |
| 47 | A0A087SKJ2 | Serine hydroxymethyltransferase | F751_4739 | 1 | 1.551 ± 0.53 | 1.871 ± 1.08 | 2.940 ± 2.44 |
| 48 | E1Z357 | Serine-glyoxylate aminotransferase | CHLNCDRAFT_33614 | 1 | 1.161 ± 0.55 | 1.958 ± 0.26 | 1.782 ± 0.41 |
(a)Mean value for the identified unique peptides in different replicates is represented. (b)Differential abundance for these candidates is also identified in DIGE. (c)These candidates are validated by Western blotting. (d)These candidates are validated by MRM. NS: Not significant.