Figure 3. Effect of inhibition of Hedgehog signaling pathway on kras^V12- and Myc-induced liver enlargement.

7 dpf fabp10+, kras+ or Myc+ larvae were treated with either 10 μM cyclopamine or 1 μM GANT61 in the presence of 10 μg/ml Dox and 2D liver size was measured based on images. (A–C) Representative images of 7 dpf fabp10+ control larvae. (D–F) Representative images of 7 dpf kras+ larvae. (G–I) Representative images of 7 dpf Myc+ larvae. (J) Quantification of liver sizes for kras+ larvae. (K) Quantification of liver sizes for Myc+ larvae. N = 20 from each groups; statistical significance: *p < 0.05, Scale bar = 20 μm.