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. 2017 Feb 8;7(4):899–911. doi: 10.7150/thno.17927

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(a) The absorbance spectra and visual color changes of TMB in different reaction systems: (1) TMB + H₂O₂, (2) BSA-AuNCs-LPs-anti-HER2 + TMB, and (3) BSA-AuNCs-LPs-anti-HER2 + TMB + H₂O₂ in PBST (25 mM Na₂HPO₄, pH 4.0, with 0.05% Tween-20) at 25 °C after 30 min incubation. (b) The absorbance spectra and visual color changes of TMB in presence of different concentrations of BSA-AuNCs-LPs-anti-HER2 after 30 min incubation. Inset: the absorption values at 652 nm depend on the concentrations of BSA-AuNCs-LPs-anti-HER2. (c, d) The peroxidase-like activity of the BSA-AuNCs-LPs-anti-HER2 is dependent on pH (c) and temperature (d).