Figure 2.

HDAC6 deficiency increases the development of IL-17-producing γδ T cells. (A) Lymphocytes isolated from the spleen of WT and HDAC6 KO mice were stimulated with PMA and ionomycin, and the expression of IFN-γ and IL-17 was analyzed in γδ T and CD4⁺ T cells. (B) Quantification of the IFN-γ- and IL-17-producing subpopulations of γδ T and CD4⁺ T cells based on flow cytometry as shown in (A). (C) Lymphocytes isolated from the spleen or lymph glands of WT and HDAC6 KO mice were stimulated with PMA and ionomycin, and the expression of IL-17 in Vγ1⁺ or Vγ4⁺ γδ T cells was analyzed by gating CD3⁺ cells. (D) Quantification of IL-17-producing subpopulations of Vγ1⁺ or Vγ4⁺ γδ T cells based on flow cytometry as shown in (C). Data are representative of three independent experiments. Error bars indicate mean ± SEM. *p < 0.05; ns, not significant.