Figure 3.

The deacetylase activity of HDAC6 is required for its regulation of IL-17 production by γδ T cells. (A) γδ T cells isolated from the spleen of WT and HDAC6 KO mice were cultured for 6 days and treated with 1 μM tubacin or an equal volume of the vehicle DMSO. IFN-γ- and IL-17-producing subpopulations of γδ T cells were then analyzed by flow cytometry. (B and C) Quantification of the percentages of IL-17-producing (B) and IFN-γ-producing (C) γδ T cells. Data are representative of three independent experiments. Error bars indicate mean ± SEM. *p < 0.05; ns, not significant.