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. Author manuscript; available in PMC: 2017 Apr 5.
Published in final edited form as: J Hepatol. 2015 Nov 30;64(4):899–907. doi: 10.1016/j.jhep.2015.11.028

Figure 2. In vivo treatment with triple siRNAs induced proliferation of hepatocytes and activation of Yap1.

Figure 2

C57BL/6 female mice were treated with triple siRNAs or dose matched si-Control. Livers were taken at Day 7, 9, 11, 13, 15, 24, 32 or 40 days after the first injection. a, Liver/body weight ratio at different time points. Error bars represent SEM, (N≥3). b, Immunohistochemical analysis of hepatocyte proliferation (Day 11). Ki-67 (grey), Phalloidin (yellow), Hoechst (blue). c, Quantification of (b). Error bars represent SEM, **, p<0.01 (N=3). d, Western blot analysis of Hippo pathway signalling. e, Triple siRNA treatment changed Yap1 localization in liver (Day 11). Yap1 (yellow), nuclei (blue). f, Expression of Yap1-dependent genes were induced by triple siRNAs (Day 11). Error bars represent SEM, **, p<0.01 (N=3).