Table 3. Oligonucleotides used to generate mutations.
His-Zta S209A amino acid serine 209 mutated to alanine | GGCTGCTGCCAAATCAGCTGAAAATGACAGGCTGCGCC; GGCGCAGCCTGTCATTTTCAGCTGATTTGGCAGCAGCC |
His-Zta S209D amino acid serine 209 mutated to glutamic acid | GGCTGCTGCCAAATCAGATGAAAATGACAGGCTGCGCC; GGCGCAGCCTGTCATTTTCATCTGATTTGGCAGCAGCC |
His-Zta K12R amino acid lysine 12 mutated to arginine | CtCGACttCtGAAGAtGtAAgAtttACACCtGACCCAtACC; GGTATGGGTCAGGTGTAAATCTTACATCTTCAGAAGTCGAG |